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KMID : 1189420030160030068
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2003 Volume.16 No. 3 p.68 ~ p.81
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Effect of Hwang-Ryeon-Hae-Dok-Tang on the Release of IL-8 in Human Nasal Mucosal Fibroblast
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Lee In-Su
Kim Hee-Taek
Lee Eun-Yong
Kim E-Hwa
Ryu Ju-Hyun
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Abstract
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It is proposed that Hwang-Ryeon-Hae-Dok-Tang may modulate the immune response on allergy or asthma. Human nasal mucosal fibroblasts are a rich source cytokines, inflammatory mediators, and chemokines. Chemokines are important for the recruitment of leukocytes to sites of infection, which is essential in host defense. Objectives : The objective of this study was to investigate the effect of Hwang-Ryeon-Hae-Dok-Tang(HH) on the release of the IL-8 chemokine in human nasal mucosal fibroblasts after stimulation with cytokines like interleukin-4(IL-4), tumor necrosis factor- (TNF- ), interferon- (lFN- ), and interle ukin-l (IL-I ). Methods : To detect the release of IL-8, enzyme-linked immunosorbent assay(ELISA) kit was performed. The cytotoxicity was measured by MTT assay. Results : HH significantly inhibited the secretion of IL-8 with a dose-dependant manner. The effective dosage did not have the cytotoxicity on human nasal mucosal fibroblasts Conclusions : Results of our study show that HH would play an important role in modulation of IL-8 in human nasal mucosal fibroblasts.
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KEYWORD
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Hwang-Ryeon-Hae-Dok-Tang , Interleukin-8(IL-8) , Human nasal mucosal fibroblasts(HNF)
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